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topo blunt cloning kit  (New England Biolabs)


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    Structured Review

    New England Biolabs topo blunt cloning kit
    Topo Blunt Cloning Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 960 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/topo+blunt+cloning+kit/NEB+PCR+Cloning+Kit/pmc12711569-404-18-22
    Average 96 stars, based on 960 article reviews
    topo blunt cloning kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Non-Homologous End Joining:

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Construct:

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Amplification:

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Polymerase Chain Reaction:

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Cloning:

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Sequencing:

    Article Title: Evidence for improved DNA repair in the long-lived bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..

    Article Title: Evidence for improved DNA repair in long-lived bowhead whale.
    Article Snippet: Denis Firsanov, Max Zacher, Xiao Tian, Todd L. Sformo, Yang Zhao, Gregory Tombline, J. Yuyang Lu, Zhizhong Zheng, Luigi Perelli, Enrico Gurreri, Li Zhang, Jing Guo, Anatoly Korotkov, Valentin Volobaev, Seyed Ali Biashad, Zhihui Zhang, Johanna Heid, Alexander Y. Maslov, Shixiang Sun, Zhuoer Wu, Jonathan Gigas, Eric C. Hillpot, John C. Martinez, Minseon Lee, Alyssa Williams, Abbey Gilman, Nicholas Hamilton, Ekaterina Strelkova, Ena Haseljic, Avnee Patel, Maggie E. Straight, Nalani Miller, Julia Ablaeva, Lok Ming Tam, Chloé Couderc, Michael R. Hoopmann, Robert L. Moritz, Shingo Fujii, Amandine Pelletier, Dan J. Hayman, Hongrui Liu, Yuxuan Cai, Anthony K. L. Leung, Zhengdong Zhang, C. Bradley Nelson, Lisa M. Abegglen, Joshua D. Schiffman, Vadim N. Gladyshev, Carlo C. Maley, Mauro Modesti, Giannicola Genovese, Mirre J. P. Simons, Jan Vijg5 ✉, Andrei Seluanov1,23 ✉ & Vera Gorbunova1,23 ✉

    Article Title: DNA repair and anti-cancer mechanisms in the longest-living mammal: the bowhead whale
    Article Snippet: Immediately after FACS, genomic DNA was isolated from cells using the QIAGEN Blood & Tissue kit. .. DSB repair sites in the NHEJ construct were amplified by PCR using Phusion polymerase (NEB), cloned using the TOPO Blunt cloning kit (NEB), and sent for Sanger sequencing. ..



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